Customization: | Available |
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CAS No.: | 75621-03-3 |
Formula: | C32h58n2o7s |
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Introduction |
CHAPS is a non denaturing zwitterionic detergent used in membrane biochemistry. Can be used to dissolve membrane proteins and interrupt protein-protein interactions |
Function |
CHAPS is a non denaturing zwitterion detergent used in membrane biochemistry. It can be used to dissolve membrane proteins and interrupt protein-protein interactions. CHAPS has a low micelle molecular weight (6150) and a high critical micelle concentration (6-10mM), so it can be removed from the sample through dialysis. Chemicalbook is also suitable for dissolving proteins in isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non denaturing (urea free) isoelectric focusing and has been found to exhibit excellent resolution for certain subcellular preparations and plant proteins. Usually, the concentration between 2-4% (w/v) is used for isoelectric focusing gel. |
Application |
Biochemical research. Amphoteric surfactant, chaps is a non denaturing zwitterionic detergent and protein lysate, which is used to dissolve membrane proteins and the interaction between lysed proteins and proteins. |
CHAPS is a zwitterionic nondenaturing detergent for solubilizing membrane proteins. It is used in the laboratory to dissolve biological macromolecules such as proteins. It acts as a non-denaturing solvent used in purification of membrane proteins. It is also useful in conjunction with nonionic detergents like Triton X-100. Further, it is used as a non-denaturing zwitterionic detergent for membrane biochemistry.
CHAPS is often used as a detergent in the solubilization and purification of membrane proteins for several advantageous reasons. CHAPS detergent is non-denaturing to membrane proteins, can solubilize proteins, disaggregate protein-protein interactions. CHAPS is also useful in ion exchange chromatography and isoelectric focusing as it is zwitterionic and does not exhibit a net charge between pH 2 to 12. It is suitable for isolation of membrane proteins with the following features such as sufficient protein solubilization capability, no denaturing or inactivation of proteins, no interference with protein activities, no precipitation at 4°C, appropriate critical micelle concentrations (CMC) and micelle size, no absorption in the UV region, no toxicity and availability of detergent detection methods.
Q&A
1.How could l get a sample?
Before we received the first order, please afford the sample cost and express fee. We wilreturn the sample cost back to you within your first order.
2.Can you make designs for us?
We have a professional design team to help our customers do design work.Both OEM and ODM orders are accepted.
3.Can we get samples from your factory?
We can provide you samples for free, you just pay express cost.
4.Whether you could make our brand on your products?
Yes. We can print your Logo on both the products and the packages if you can meet our MOQ
How can you provide us high quality products?
5.We have a professional QC team, every product is shipped after strictly examination.
What is your lead time?
Quantity about 10000 pcs is 15-20 days ,small orders are about one week.
Huge orders to be neqotiated.
6.What is your payment?
We accept T/T.L/C.Westem union or to be negotiated.Don't worry about anything, if you have any problems, please feel free to contact us