Customization: | Available |
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Type: | Pharmaceutical Intermediates |
Appearance: | Powder |
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Apperance: | White Crystal Powder | Purity: | >99% |
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Molecular Weight: | 410.47 | Molecular Formula: | C19H23N4NaO3S |
Grade: | Analytical Grade | Storage Condition: | Store at 2-10 ºC,away from light and moisture |
Transportation Condition: | Store at 2-10 ºC | CAS: | 115871-18-6 |
The chromogenic substrate DA-67 is an intermediate metabolite in the constant component analysis and detection of organisms. Its CAS number is 115871-18-6, and its appearance is white powder. It is worth noting that its storage requires temperature, and it needs to be stored at 2-10 ºC. Therefore, if it is transported at normal temperature, it needs to be placed in ice bags and packed in foam boxes.
Method for Quantitative Determination of Hydrogen Perexide Using DA-67
1. Prepare 4.08 mg of DA-67 powder, 1 mL100 units/mL of peroxidase, 0.1mol/L, pH=7, Triton X-100 0.5% PIPES buffer;
2. Place the prepared DA-67 powder and peroxidase into PIPES buffer and bring to a constant volume of 100 mL (DA67 100 μ Mol/L solution);
3. Take 3ml of the prepared solution and mix it with 10 μ Mix the sample solution L (H202: 0-5 mmol/L);
4. Incubate at 37 ºC for 5 minutes;
5. Measure the absorbance at 666 nm using a spectrophotometer, and use the absorbance difference of the blank (solution mixed with distilled water to replace the sample solution) as the detection value. Use a separately prepared calibration curve to determine the content in the sample.
The Application Principle of DA-67
When used in combination with peroxidase, DA-67 exhibits high sensitivity to hydrogen perexide and is a water-soluble oxidative chromogenic reagent. Compared to traditional color reagents, it not only has the characteristics of low absorbance and high sensitivity, but also has better water solubility compared to other color reagents DA-67. At a concentration of more than 1 mmol/L, 10 milligrams can be dissolved in 25 milliliters of water. It is compatible with the above advantages and requires a simple colorimetric method to obtain results quickly, greatly increasing detection efficiency. Therefore, DA-67 is commonly used in the analysis of biological component samples.
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Quantity about 10000 pcs is 15-20 days ,small orders are about one week.
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